基因枪介导的老芒麦遗传转化体系的建立

川草2号老芒麦(Elymus sibiricus)是青藏高原地区治理荒漠化和建设高产人工草地的主要栽培草种。用川草2号老芒麦5种外植体诱导愈伤组织，经分化测试，仅幼穗愈伤组织能分化再生。以当代培养25天和35天的结构致密坚硬的幼穗愈伤组织为受体，分别进行农杆菌侵染和基因枪转化，结果只有基因枪能转化成功。在基因枪转化过程...

Establishment of Biolistic Mediated Transformation System for Elymus sibiricus

Elymus sibiricus cv. ‘Chuancao No.2’ is the main cultivated grass species for desertification control and construction of high-yield and high-quality pasture in northwest Sichuan Plateau. In this study, we tested five explants of E. sibiricus cv. ‘Chuancao No.2’ for callus induction, and found that only inflorescence calli were able to differentiate and regenerate. The calli of inflorescence with dense and hard structure cultured for 25 d and 35 d were used for Agrobacterium and biolistic mediated transformation respectively. The results showed that only biolistic-mediated transformation could produce positive transgenic calli of ‘Chuancao No.2’. In the process of biolistic-mediated transformation, the calli was pretreated in two ways: hyperosmotic culture and filter paper drying. The results revealed that the transformation efficiency of filter paper drying was higher than that of hyperosmotic treatment. For the inflorescence callus after 25 d induction, the transformation efficiency under the condition of 2 h drying of filter paper was highest which reached about 40%. In short, we applied the biolistic technology in ‘Chuancao No.2’ for the first time and successfully obtained the positive transgenic inflorescence calli. This work will lead to establishment of the robust transformation system for E. sibiricus in future.