| 响应面法优化飞龙掌血中总生物碱的提取工艺 |
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| 引用本文： | 冯慧霞,陈桂林,郭明全.响应面法优化飞龙掌血中总生物碱的提取工艺[J].植物科学学报,2021,38(6):831-838. |
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| 作者姓名： | 冯慧霞 陈桂林 郭明全 |
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| 作者单位： | 1. 中国科学院武汉植物园, 中国科学院植物种质创新与特色农业重点实验室, 武汉 430074;
2. 中国科学院中-非联合研究中心, 武汉 430074;
3. 中国科学院药物创新研究院, 上海 201203;
4. 中国科学院大学, 北京 100049 |
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| 基金项目： | 湖北省对外科技合作技术创新专项（2017AHB054）；湖北省自然科学基金项目（2019CFB254）；中国科学院青年创新促进会项目（2020337）。 |
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| 摘 要： | 以飞龙掌血（Toddalia asiatica（L.）Lam.）的根为实验材料，以总生物碱的含量为检测指标，应用响应面法对飞龙掌血中总生物碱的提取工艺进行了优化。结果显示，当提取条件为乙醇体积分数74.76%、料液比20∶1和提取时间32.87 min时，飞龙掌血提取物中总生物碱的理论含量值可达11.09 mg/g，总生物碱含量达到最高值。按照最佳提取工艺条件进行验证，3次平行实验的均值为（10.74 ±0.18）mg/g，与理论值差异较小，表明利用响应面法来优化飞龙掌血中总生物碱的最佳提取工艺模型拟合良好，具有一定实用价值。
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| 关 键 词： | 飞龙掌血 总生物碱 提取工艺 响应面法 |
Optimization of the extraction process of total alkaloids in Toddalia asiatica (L.) Lam. by response surface methodology |
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| Feng Hui-Xia,Chen Gui-Lin,Guo Ming-Quan.Optimization of the extraction process of total alkaloids in Toddalia asiatica (L.) Lam. by response surface methodology[J].Plant Science Journal,2021,38(6):831-838. |
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| Authors: | Feng Hui-Xia Chen Gui-Lin Guo Ming-Quan |
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| Institution: | 1. CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;
2. Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, 437004, China;
3. Innovation Academy for Drug Discovery and Development, Chinese Academy of Sciences, Shanghai 201203, China;
4. University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | In this study, the root of Toddalia asiatica (L.) Lam. was used as the experimental material and the content of total alkaloids was considered as the detection index. The optimum extraction conditions of total alkaloids in T. asiatica roots were investigated using response surface methodology (RSM). The optimum extraction conditions were: ethanol concentration of 74.76%, material-solvent ratio of 20∶1, and extraction time of 32.87 min, with a theoretical total alkaloid content of 11.09 mg/g. According to the optimum extraction conditions, the average value of three parallel experiments was (10.74 ±0.18) mg/g, which showed little difference from the theoretical content. Thus, the optimum extraction conditions of total alkaloids in T. asiatica obtained by the RSM were accurate and reliable, and this mathematical model was well fitted and of practical value. |
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| Keywords: | Toddalia asiatica Total alkaloids Extraction Response surface methodology |
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