Characterization of a cDNA encoding ricin E,a hybrid ricin-Ricinus communis agglutinin gene from the castor plant Ricinus communis |
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| Authors: | Beth F Ladin Elizabeth E Murray Amy C Halling Kevin C Halling Nanda Tilakaratne George L Long L L Houston Robert F Weaver |
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| Institution: | (1) Department of Biochemistry, University of Kansas, 66045 Lawrence, KS, USA;(2) Present address: Henkel Corporation, Santa Rosa, CA;(3) Present address: Agrigenetics Advanced Research Laboratory, Madison, WI;(4) Eli Lilly and Company, Indianapolis, IN;(5) Present address: Cetus Corporation, Emeryville, CA |
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| Abstract: | Two classes of ricin cDNA clones have been identified and sequenced. The cDNA clone pBL-1 closely matches in nucleotide sequence the ricin genomic clone pAKG previously described by Halling et al., 1985 (Nucl. Acids Res. 13:8019). A second group of cDNA clones, represented by pBL-3, encode a hybrid protein (ricin E), having a ricin-like A chain and N-terminal half of the B chain and an RCA (Ricinus communis agglutinin)-like C-terminal half of the B chain. |
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