| 鹦鹉热衣原体主要外膜蛋白基因序列的扩增、克隆和原核表达 |
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| 引用本文： | 刘向伟,端青,张浩杰,王效义,何君,张国珍.鹦鹉热衣原体主要外膜蛋白基因序列的扩增、克隆和原核表达[J].微生物学免疫学进展,2002,30(2):32-34. |
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| 作者姓名： | 刘向伟 端青 张浩杰 王效义 何君 张国珍 |
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| 作者单位： | 军事医学科学院微生物流行病研究所,北京,100071 |
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| 摘 要： | 主要外膜蛋白在鹦鹉热衣原体感染过程中起主要作用。扩增了主要外膜蛋白基因，克隆入pGEM-T和pET32a( )，经PCR筛选和酶切鉴定，进行诱导表达和重组蛋白的纯化与复性研究，为进一步进行鹦鹉热衣原体的诊断试剂和疫苗研究创造了条件。
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| 关 键 词： | 鹦鹉热衣原体 主要外膜蛋白 克隆 |
| 文章编号： | 1005-5673(2002)-02-0032-03 |
| 修稿时间： | 2001年8月10日 |
Amplification, cloning and prokaryotic expression of gene of major outer-membrane protein of Chlamydia psittaci |
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| LIU Xiang wei,DUANG Qing,ZHANG Hao jie,et al..Amplification, cloning and prokaryotic expression of gene of major outer-membrane protein of Chlamydia psittaci[J].Progress In Microbiology and Immunology,2002,30(2):32-34. |
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| Authors: | LIU Xiang wei DUANG Qing ZHANG Hao jie |
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| Abstract: | Major outer membrane protein plays a key role in the infection of Chlamydia psittaci.In this report,the gene of the major outer membrane protein was amplified and cloned into plasmid pGEM T and pET32a(+).The positive strains were determined by PCR and enzymic digestion and then induced.The purifiation and renaturation were further carried out to the purpose of application of the recombinant protein. |
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| Keywords: | Chlamydia psittaci Major outer membrane protein Cloning |
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