Isolation of sheep anterior pituitary messenger RNA and its translation in a heterologous cell-free system |
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Authors: | M S K Prasad P R Adiga |
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Institution: | (1) Department of Biochemistry, Indian Institute of Science, 560 012 Bangalore;(2) Present address: Department of Biological Sciences, Oakland University, Oakland, Michigan, USA |
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Abstract: | A preparation rich in the specific messenger RNA involved in the synthesis of prolactin from sheep anterior pituitary glands
was obtained by employing both the immunochemical and affinity techniques. A dose-dependent and efficient stimulation of protein
synthesis by the isolated total pituitary RNA as well as poly (A) rich RNA were achieved with the reticulocyte system. The
synthesis of prolactin as one of the translational products of this cell-free system was established by specific immunoprecipitation
followed by resolution on sodium dodecyl sulphate polyacrylamide gel electrophoresis. |
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Keywords: | Pituitary RNA oligo (dT)-cellulose prolactin-mRNA reticulocyte ysate immunoprecipitation |
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