| d－生物素代替玉米浆发酵生产谷氨酸的研究 |
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| 引用本文： | 梁新梅,孙吉臻,张跃庆,王丽谦,董金兰,徐娉婷. d－生物素代替玉米浆发酵生产谷氨酸的研究[J]. 生物技术, 1996, 0(2) |
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| 作者姓名： | 梁新梅 孙吉臻 张跃庆 王丽谦 董金兰 徐娉婷 |
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| 作者单位： | 北方味精厂，哈尔滨师范大学生物系，黑龙江省科学院应用微生物所 |
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| 摘 要： | 用ｄ－生物素代替玉米浆的摇瓶发酵实验，筛选出高产酸菌株，测定了ｄ－生物素的亚运量可变范围。比较了单一玉米浆、单一ｄ－生物素与两者各半量发酵结果及不同接种量对产酸的影响。采用ｄ－生物素代替半量玉米浆的中糖发酵工艺应用于生产。结果证明此工艺可行，降低了成本，提高了产酸率，平均产酸率达７．８１％，平均转化率达５３．２％。
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| 关 键 词： | d－生物素，玉米浆，谷氨酸，发酵 |
Study on SUbstitution of d-Biotin for Corn Steep Liquor in Fermentative Production of Glutamic Acid |
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| Liang Xinmei,Shun Jizhen, Zhang Yueqing,Wang Liqian. Study on SUbstitution of d-Biotin for Corn Steep Liquor in Fermentative Production of Glutamic Acid[J]. Biotechnology, 1996, 0(2) |
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| Authors: | Liang Xinmei Shun Jizhen Zhang Yueqing Wang Liqian |
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| Abstract: | With the fermentative experiments of shake flask in which corn steep liquor was replaced by d-biotin,the high acid-produced strains were selected and the variable range of sub opportune amount of d-biotin was determined.By comparing the fermentative results in using single corn steep liquor,Singte d-biotin and both in half amount.and the effects of different inoculating amount on producing acid,the medium-sugar fermentative technology of substitution of d-biotin for half-amount corn steep liquor was used in production.This technology has been proved feasible by its decreasing cost and increasing acid-produced rate.Average acid-produced rate is 7.81 % and average transformation is 53.2 %. |
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| Keywords: | d-biotin Corn steep liquor Glutamic acid Fermentation |
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