| RP-HPLC法测定青海产四种秦艽中獐牙菜苦苷的含量 |
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| 引用本文： | 张兴旺,于瑞涛,梅丽娟,邵赟,陶燕铎.RP-HPLC法测定青海产四种秦艽中獐牙菜苦苷的含量[J].中国野生植物资源,2009,28(5):51-53. |
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| 作者姓名： | 张兴旺 于瑞涛 梅丽娟 邵赟 陶燕铎 |
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| 作者单位： | 1. 中国科学院西北高原生物研究所,青海,西宁,810008;中国科学院研究生院,北京,100049
2. 中国科学院西北高原生物研究所,青海,西宁,810008 |
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| 基金项目： | 科技部国家科技支撑计划 |
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| 摘 要： | 建立了秦艽中獐牙菜苦苷含量测定的RP-HPLC方法。色谱条件：采用ZorbaxEclipseXDB-C18柱（150mm×4.6mm,5μm）,流动相：甲醇-0.03%磷酸水溶液梯度洗脱（0～25min：15%～23%;25～30min：23%～30%）,流速：1mL/min,柱温：25℃,检测波长240nm,獐牙菜苦苷在0.20～1.8μg范围内成良好的线性关系,r=0.9999,回收率RSD=1.32%。对青海产4种秦艽中獐牙菜苦苷的含量进行了定量分析,测定结果：小秦艽中獐牙菜苦苷的含量最高为：0.62%,麻花秦艽与管花秦艽的含量没有显著差异,分别为：0.43%,0.45%,簧管秦艽的含量最低为：0.32%。
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| 关 键 词： | RP-HPLC 秦艽 獐牙菜苦苷 |
Determination of Swertiamarin in Four Kinds of Gentiana straminea Maxim.in Qinghai by RP-HPLC |
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| Zhang Xingwang,Yu Ruitao,Mei Lijuan,Shao Yun,Tao Yanduo.Determination of Swertiamarin in Four Kinds of Gentiana straminea Maxim.in Qinghai by RP-HPLC[J].Chinese Wild Plant Resources,2009,28(5):51-53. |
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| Authors: | Zhang Xingwang Yu Ruitao Mei Lijuan Shao Yun Tao Yanduo |
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| Institution: | Zhang Xingwang, Yu Ruitao, Mei Lijuan , Shao Yun, Tao Yanduo(1. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China; 2. Graduate School of Chinese Academy of Sciences, Beijing 100049, China) |
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| Abstract: | A method was established for the determination of swertiamarin by RP - HPLC. Experiments were performed on Zorbax Eclipse XDB - C18 （4.6 mm × 250 mm,5 μm） column, with CH3OH - 0. 03%H3PO4（0-25 min： 15% -23% ;25 -30 rain：23%- 30% ）as mobile phase at a flow rate of 1.0 mL/min. The column temperature was 25 ℃ and the determination wavelength was 240 nm. The linear range was 0.20 μg - 1.8 μg（ r = 0.999 9） and the average recovery was 98.6% （RSD = 1.32% ）. The contents of swertiamarin were detected, and the results were relevant to the source. The content of swertiamarin of Gentiana dahurica Fisch. was 0.62%, the content of Gentiana straminea Maxim. and Gentiana siPhonantha Maxim. ex Kusnez are 0.43% ,0.45%, Gentiana officinalis H. Smith is 0.32%. |
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| Keywords: | RP-HPLC |
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