Expression of the Mouse Serotonin Receptor (5HT1c) cDNA in Cultured Insect Cells |
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| Authors: | Beljelarskaya S. N. Sutton F. |
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| Affiliation: | (1) Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia;(2) University of South Dakota, South Dakota, 57007, United States |
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| Abstract: | Baculovirus-mediated cloning and expression of the mouse serotonin receptor (5HT1c) cDNA in insect cells was proposed to create an alternative to the oocyte-based system commonly employed in electrophysiological studies of ionic channels. A recombinant bacmid was constructed, and the 5HT1c cDNA was transferred into the AcNPV genome to yield a recombinant baculovirus. Infected insect Sf9 cells produced recombinant 5HT1c. |
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| Keywords: | serotonin receptor ionic channels baculoviruses insect cells Escherichia coli oocytes gene expression recombinant proteins |
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