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Investigation of the apoptotic way induced by digallic acid in human lymphoblastoid TK6 cells |
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| Authors: | Wissem Bhouri Jihed Boubaker Ines Skandrani Kamel Ghedira Leila Chekir Ghedira |
| Affiliation: | 1. Laboratoire de biologie Cellulaire et Mol??culaire, Facult?? de Medecine Dentaire Monastir, Rue Avicenne, Monastir, 5000, Tunisia 2. Unit?? de pharmacognosie 99/UR/07, Facult?? de pharmacie Monastir, Rue Avicenne, Monastir, 5000, Tunisia 3. Faculty of Dental Medicine, Rue Avicenne, Monastir, 5019, Tunisia |
| Abstract: | ABSTRACT: BACKGROUND: The digallic acid (DGA) purified from Pistacia lentiscus. L fruits was investigated for its antiproliferative and apoptotic activities on human lymphoblastoid TK6 cells. METHODS: We attempt to characterize the apoptotic pathway activated by DGA. Apoptosis was detected by DNA fragmentation, PARP cleavage and by evaluating caspase activities. RESULTS: The inhibition of lymphoblastoid cell proliferation was noted from 8.5 mug/ml of DGA. The induction of apoptosis was confirmed by DNA fragmentation and PARP cleavage. We have demonstrated that DGA induces apoptosis by activating the caspase-8 extrinsic pathway. Caspase-3 was also activated in a dose dependent manner. CONCLUSION: In summary, DGA exhibited an apoptosis inductor effect in TK6 cells revealing thus its potential as a cancer-preventive agent. |
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