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In vitro Induction of primary antibody responses to particulate and soluble protein antigens in T cell—replaced murine spleen cell cultures
作者姓名:LuKun
作者单位:ShanghaiInstituteofCellBiology,AcademiaSinica,Shanghai,200031,China
摘    要:Specific antibody responses could be induced in serumfree condition.Specific anti-SRBC or anti-SRBC ghost antibody were induced from anti-Thy treated (T-depleted) murine spleen cells in serum-free culture in the presence of Con A conditioned medium.This induction system may facilitate the study of lymphokine functions on antigen triggered B cells. In T cell-replaced cultures,the antibody responses of B cells could be successfully induced when soluble SRBC membrane proteins were used as antigens.It thus indicates that antigen together with lymphokines are sufficient to drive B cells to become antibody secreting cells in the absence of T cells.The T cell-replaced system provides a more stable way for in vitro immunization and may be applied to monoclonal antibody production when in vivo immunization is difficult to be carried out.

关 键 词:B细胞  鼠脾细胞培养物  T细胞替代  抗体反应  可溶性蛋白抗原  体外诱导

In vitro Induction of primary antibody responses to particulate and soluble protein antigens in T cell-replaced murine spleen cell cultures
LuKun.In vitro Induction of primary antibody responses to particulate and soluble protein antigens in T cell-replaced murine spleen cell cultures[J].Cell Research,1990,1(1):23-33.
Authors:Lu Kun
Institution:Lu Kun Shanghai Institute of Cell Biology,Academia Sinica,Shanghai,200031,China.
Abstract:Specific antibody responses could be induced in serum-free condition. Specific anti-SRBC or anti-SRBC ghost antibody were induced from anti-Thy treated (T-depleted) murine spleen cells in serum-free culture in the presence of Con A conditioned medium. This induction system may facilitate the study of lymphokine functions on antigen triggered B cells. In T cell-replaced cultures, the antibody responses of B cells could be successfully induced when soluble SRBC membrane proteins were used as antigens. It thus indicates that antigen together with lymphokines are sufficient to drive B cells to become antibody secreting cells in the absence of T cells. The T cell-replaced system provides a more stable way for in vitro immunization and may be applied to monoclonal antibody production when in vivo immunization is difficult to be carried out.
Keywords:B cell maturation  lymphokines  in vitro immunization  
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