Protonic and cationic changes during cyclical cation transport driven by electron transfer |
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| Authors: | D E Green H D Vande Zande |
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| Affiliation: | 1. Department of Biological Sciences Wayne State University, Detroit, MI 48202 USA;2. Department of Chemistry Wayne State University, Detroit, MI 48202 USA |
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| Abstract: | A method is described for the subcellular fractionation of goldfish xanthophores. The procedure produces relatively pure fractions of caroteniod droplets, pterinosomes, cytosol and what appears to be plasma membrane. The presence of a distinct pattern of proteins is shown to be associated with the carotenoid droplets. Treatment of the xanthophores with ACTH affects the buoyant density of some carotenoid droplets and stimulates the phosphorylation of a polypeptide associated with the carotenoid droplets. |
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| Keywords: | ACTH adrenocorticotropin cAMP adenosine 3′,5′-cyclic monophosphate MSH melanocyte stimulating hormone SDS sodium dodecyl sulfate |
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