Effects of methyl jasmonate (MeJA) on the dark-induced senescence in oat (Avena sativa L.) leaf segments |
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Authors: | Chang Yong Hoon Myeong Min Lee Sun Hi Lee Yong Chung |
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Institution: | (1) Department of Biology, Yonsei University, Sinchon 134, 120-749 Seoul, Korea;(2) Institute for Environmental Research, Yonsei University, Sinchon 134, 120-749 Seoul, Korea |
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Abstract: | To investigate the relationship between methyl jasmonate (MeJA) and ethylene in leaf senescence, we studied the effects of
MeJA on ethylene production and ethylene biosynthetic enzyme activities in oat(Avena sativa L.) leaf segments incubated in darkness. MeJA promoted dark-induced senescence judged from the contents of chlorophyll and
protein, and increased ethylene production 6 times of the control. MeJA also increased the activities of ethylene biosynthetic
enzymes, 1-aminocyclopropane carboxylic acid (ACC) synthase and ACC oxidase as compared to control. In MeJA-treated leaf segments,
ACC synthase activity reached its maximum level at 24 h of incubation and ACC oxidase activity peaked at 6 h of incubation.
Aminoethoxyvinylglycine (AVG) and Co2+, inhibitors of ACC synthase and ACC oxidase respectively, reduced MeJA-induced ethylene production. They also delayed leaf
senescence that was promoted by the treatment of MeJA. From these results, we can suggest that MeJA increased the activities
of ACC synthase and ACC oxidase, these increased activities lead to increase in ethylene production and this increased ethylene
production might promote dark-induced leaf senescence. |
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Keywords: | methyl jasmonate leaf senescence ethylene production ACC synthase ACC oxidase cobalt ion AVG |
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