Characterization of nuclear localization and nuclear export signals of yeast actin-binding protein Pan1 |
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Authors: | Kamińska Joanna Sedek Magdalena Wysocka-Kapcińska Monika Zo?adek Teresa |
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Institution: | Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 01-106 Warsaw, Poland. kaminska@ibb.waw.pl |
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Abstract: | Pan1 is an actin patch-associated protein involved in endocytosis. Our studies revealed that in oleate-grown cells Pan1 is located in the nucleus as well as in patches. One of three putative nuclear localization signals (NLS) of Pan1, NLS2, directed beta-galactosidase (beta-gal) to the nucleus. However, GFP-Pan1(886-1219), containing NLS2, was found in the cytoplasm indicating that it may contain a nuclear export signal (NES). A putative Pan1 NES, overlapping with NLS3, re-addressed NLS(H2B)-NES/NLS3-beta-gal from the nucleus to the cytoplasm. Inactivation of the NES allowed NLS3 to be effective. Thus, Pan1 contains functional NLSs and a NES and appears to shuttle in certain circumstances. |
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Keywords: | NES nuclear export signal NLS nuclear localization signal |
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