Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting |
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Authors: | Takeuchi Tomonori Nomura Takanori Tsujita Mika Suzuki Misao Fuse Toshimitsu Mori Hisashi Mishina Masayoshi |
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Affiliation: | Department of Molecular Neurobiology and Pharmacology, Graduate School of Medicine, University of Tokyo, Japan. |
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Abstract: | We constructed an expression vector of Flp recombinase modified by adding a nuclear localization signal. Injection of the expression vector into fertilized eggs of the C57BL/6 strain yielded transgenic mouse lines expressing the Flp recombinase transgene in the testis. We crossed the transgenic mice to reporter mice carrying the neomycin phosphotransferase gene flanked by target sites of Flp recombinase. Examination of the deletion of the neomycin phosphotransferase gene in the progeny showed that Flp-mediated recombination took place efficiently in vivo in FLP66 transgenic mouse line. These results suggest that the Flp recombinase system is effective in mice and in combination with the Cre recombinase system extends the potentials of gene manipulation in mice. One of the useful applications of FLP66 transgenic mouse line is the removal of marker genes from mice manipulated for the conditional gene targeting with the Cre/loxP system in the pure C57BL/6 genetic background. |
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Keywords: | Flp recombinase Frt site Transgenic mouse Conditional gene targeting C57BL/6 strain Cre/loxP system |
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