Trials for the co-expression of the merozoite surface protein-1 and circumsporozoite protein genes of Plasmodium vivax |
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Authors: | Lee Choonghee Chung Kyung Won Kim Tong-Soo Choi Kyung-Mi Choi Yien Kyoung Chung Nam-Jun Rhie Ho-Gun Lee Ho-Sa Lee Sung-Jae Lee Hyeong-Woo |
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Affiliation: | aInstitute of Global Environment and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea;bDepartment of Parasitology, College of Medicine, Inha University, Incheon 405-751, Republic of Korea;cDivision of Malaria and Parasitic Diseases, National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongwon-gun 363-951, Republic of Korea;dDepartment of Pathology, University of Florida, J-566, 1600 SW Archer Road, Gainesville, FL 32610, USA |
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Abstract: | Merozoite surface protein-1 (MSP-1), a major asexual blood stage antigen, and circumsporozoite protein (CSP), a component of sporozoites that includes a Plasmodium vivax B-cell epitope, are strong candidates for use in a malaria vaccine. A chimeric recombinant gene containing portions of both msp-1 and csp from P. vivax separated by Pro-Gly linker motif was generated. The construct gene was named mlc (msp-1, linker, and csp). The MLC chimeric recombinant protein had a molecular weight of approximately 25 kDa when expressed in Escherichia coli, as determined with sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) analysis. The purified chimeric protein reacted with the sera of patients infected with P. vivax but not with the sera of uninfected patients according to western blot analysis. The chimeric protein reacted well with sera of malaria patients (109/115, 94.78%) as assessed with enzyme-linked immunosorbent assay (ELISA). BALB/c mice that were orally immunized with the MLC chimeric recombinant protein successfully produced antigen-specific antibodies. Additionally, levels of the Th1-associated cytokines IL-12(p40), TNF-α, and IFN-γ were significantly increased in the spleens of the BALB/c mice. Therefore, the E. coli-expressed MLC chimeric recombinant protein might be used as a valuable vaccine candidate for oral immunization against vivax malaria. |
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Keywords: | Plasmodium vivax Merozoite surface protein-1 Circumsporozoite protein Vaccine |
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