Binding of gliadin to lymphoblastoid,myeloid and epithelial cell lines |
| |
Authors: | M A Farré Castany P Kocna H Tlaskalová-Hogenová |
| |
Institution: | (1) Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic;(2) Laboratory of Gastroenterology, Faculty of General Medicine, Charles University, 121 11 Prague 1, Czech Republic |
| |
Abstract: | The aim of our work was to investigate thein vitro reactivity of gliadin peptides of natural and synthetic origin with various cell lines. We have found that all tested cell
lines of human, mouse and rat origin were agglutinated by enzymically digested gliadin (peptic-tryptic-and peptic-tryptic
pancreatic digest of α-gliadin) in a concentration dependent manner. In order to test the specificity of binding, inhibition
studies were performed using a panel of sugars as well as natural and synthetic peptides derived from gliadin. We have found
that among twelve tested sugars only fetuin and phosphomannan were able to inhibition by gliadin peptides and most of the
saccharides suggests that agglutinating activity of gliadin is the result of a nonspecific binding of gliadin to the cell
membrane.
Dedicated to Professor J. Šterzl on the occasion of his 70th birthday |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|