ABC-ELISA检测抗HBc的方法及其在临床和流行病学中的初步应用

应用生物素与抗生物素系统酶联免疫吸附试验建立了检测乙型病毒性肝炎患者血清中抗HBc的ABC-ELISA方法并与普通ELISA法进行了比较。结果表明:本法敏感性较普通ELISA法高4倍,阳性检出率提高了42.42％,且具有较好的重复性。将乙肝不同抗原、抗体进行替代试验和用纯化抗HBc-lgG进行抑制试验,证明本法有较高的特异性。将本法制备成试剂盒,并与上海市传染病院,静华公司及科华公司生产的普通ELISA试剂盒进行了比较,结果本试剂盒阳性检出率分别提高了25.00％,45.28％和30.77％。经上海市三个医院临床标本试验表明,本法具有快速、敏感、特异及稳定等优点。从而为抗HBc的检测提供了一种敏感的方法。

A Method for Detection of Anti-HBc by ABC-ELISA and Its Epidemiological and Clinical Application

The method of using ABC-ELISA to measure anti-HBC in the serum of patients with hepatitis B was developed. The experimental results indicated that the sensitivity of ABC-ELISA was five times higher than that of the conventional ELISA. On the average, the positive detection rate of anti-HBc by means of ABC-ELISA increased by 42.42% over the one by using conventional ELISA. Repeated experiments showed good reproductivity. Alternative tests using various antigens and antibodies of HBV and blockage test using purified antiHBc indicated that ABC-ELISA featured good specificity. ABC-ELISA kit box for detecting anti-HBc had been campared with three conventional ELISA boxes The results indicated that the positive rate by using ABC-ELISA reagent box selectively increased by 25.00%, 45.28% and 30.77% over the one by using three boxes produced from Shanghai's Infectious Disease Hospital, Jinghua Company and Kehua Company. ABC-ELISA test with clinical serum specimens by three hospitals in Shanghai demons-trated that this method had the following features: rapidity, sensitivity, specificity and good reproductivity. ABC-ELISA had provided a reliable new method for the detection of anti-HBc.