A dimeric creatine kinase from a sponge: implications in terms of phosphagen kinase evolution |
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Authors: | Ellington W R |
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Institution: | Department of Biological Science, Florida State University, Tallahassee 32306-4370, USA. elling@bio.fsu.edu |
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Abstract: | This study demonstrates conclusively that tissues of the sponge Tethya aurantia contain significant creatine kinase (CK) activity. This CK was purified and analyzed with respect to a number of physico-chemical properties. Size exclusion chromatography and denaturing gel electrophoresis analyses showed that this enzyme is dimeric. The sequences of several Lys-C endoproteinase peptides from Tethya CK are consistent with this enzyme being a member of the phosphagen kinase family and a true CK. CK in higher organisms exists in a variety of quaternary structure forms--dimer, octamer and large monomer consisting of a three contiguous CK domains. The present results indicate that CK evolved very early in metazoan evolution and that the dimeric structure preceded other subunit association forms. |
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