Formation of active nitrite reductase (cytochromecd
1) in the strainParacoccus denitrificans HUUG25 |
| |
Authors: | Ivana Mafchová Igor Ku?era |
| |
Institution: | (1) Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 2, CZ-61137 Brno, Czech Republic |
| |
Abstract: | Strain HUUG25 ofParacoccus denitrificans has been frequently thought to be devoid of allc-type cytochromes. We show here by means of enzymological and immunological techniques that the mutant synthesizes active
nitrite reductase (cytochromecd
1) upon prolonged exposure to microoxic conditions. The synthesis occurred faster in the presence of exogenous hemin. The time
pattern of 5-aminolevulinate synthase activity was also altered by the mutation. These findings suggest a defective regulation
of heme supply to the site of nitrite reductase assembly in the periplasm. |
| |
Keywords: | Nitrite reductase Cytochromec 5-Aminolevulinate synthase Heme Strain HUUG25 Paracoccus denitrificans |
本文献已被 SpringerLink 等数据库收录! |