| 丝羽乌骨鸡微卫星标记多重PCR体系的建立与初步应用 |
| |
| 引用本文： | 孟祥军,朱庆,张明亚,李亮,刘益平. 丝羽乌骨鸡微卫星标记多重PCR体系的建立与初步应用[J]. 遗传, 2008, 30(4): 521-526. DOI: 10.3724/SP.J.1005.2008.00521 |
| |
| 作者姓名： | 孟祥军 朱庆 张明亚 李亮 刘益平 |
| |
| 作者单位： | 1. 四川农业大学动物科技学院, 雅安 625014;2. 杭州市余杭区畜牧兽医局, 余杭 311100;3. 杭州汇诚生命科学研究有限公司, 萧山 311251 ; |
| |
| 摘 要： | 采用多重PCR的方法, 对其反应条件进行优化, 以获得用于丝羽乌骨鸡遗传分析的稳定PCR体系。18个微卫星标记共获得5个扩增效果理想的组合, 其中3个为四重PCR, 2个为三重PCR。将扩增产物进一步合并为3组, 用ABI 3100-Avant遗传分析仪检测。实验结果表明, 所选用的微卫星标记表现出丰富的多态性, 可成功地应用于丝羽乌骨鸡的遗传分析。
|
| 关 键 词： | 丝羽乌骨鸡 微卫星标记 多重PCR |
| 收稿时间： | 2007-09-28 |
| 修稿时间： | 2007-09-28 |
Establishment and preliminary application of multiplex PCR for detecting the microsatellite markers in silkies |
| |
| MENG Xiang-Jun,ZHU Qing,ZHANG Ming-Ya,LI Liang,LIU Yi-Ping. Establishment and preliminary application of multiplex PCR for detecting the microsatellite markers in silkies[J]. Hereditas, 2008, 30(4): 521-526. DOI: 10.3724/SP.J.1005.2008.00521 |
| |
| Authors: | MENG Xiang-Jun ZHU Qing ZHANG Ming-Ya LI Liang LIU Yi-Ping |
| |
| Affiliation: | 1. College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014, China;2. Yuhang District Animal Husbandry and Veterinary Bureau, Yuhang 311100, China;3. Hangzhou Alltrust Life Science Research Co., Ltd., Xiaoshan 311251, China ; |
| |
| Abstract: | In order to obtain the stable PCR combinations for silkies genetic analysis, multiplex PCR was used and its reaction condition was optimized. Five combinations of multiplex PCR with good effects were obtained from eighteen microsatellite markers. Three of them were quadruplex-PCRs, and two were triplex-PCRs. The products of multiplex PCR were further combined into three sets for the electrophoresis on ABI 3100-Avant Genetic Analyzer. The results suggested that the eighteen microsatellite markers could be successfully applied to the silkies genetic analysis. |
| |
| Keywords: | |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《遗传》浏览原始摘要信息 |
|
点击此处可从《遗传》下载全文 |
|
