Cellular expression of monocarboxylate transporters in the female reproductive organ of mice: implications for the genital lactate shuttle |
| |
Authors: | Takuya Kuchiiwa Junko Nio-Kobayashi Hiromi Takahashi-Iwanaga Takaji Yajima Toshihiko Iwanaga |
| |
Institution: | (1) Laboratory of Histology and Cytology, Graduate School of Medicine, Hokkaido University, Kita 15-Nishi 7, Sapporo 060-8638, Japan;(2) Meiji Dairies Research Chair, Creative Research Initiative “Sousei” (CRIS), Hokkaido University, Kita 21-Nishi 10, Sapporo 001-0021, Japan; |
| |
Abstract: | The present study examined the cellular localization of monocarboxylate transporters (MCTs), glucose transporters (GLUTs),
and some glycolysis-related molecules in the murine female genital tract to demonstrate existence of lactate/pyruvate-dependent
energy systems. MCT1, a major MCT subtype, was localized selectively in the ovarian granulosa, oviductal-ciliated cells, and
vaginal epithelium; all localizations were associated with intense expressions of glycolytic enzymes. MCT1 was localized in
the cell membrane of granulosa cells, including fine processes extending from cumulus cells toward oocytes. The cumulus cells
and oocytes showed intense signals for lactate dehydrogenase (LDH)-A and -B, respectively. The basolateral membrane of oviductal-ciliated
cells expressed MCT4 as well as MCT1, while adjacent non-ciliated cells contained an intense immunoreactivity for aldolase-C,
a glycolytic enzyme. The expression of GLUTs in the ovary was generally weak with an intense expression of GLUT1 only in some
vascular endothelia. The oviductal epithelium expressed GLUT1 and GLUT3, respectively, in the basolateral and apical membrane
of non-ciliated cells. In the vagina, the basal layers of epithelium were immunolabeled for MCT1 with the entire length of
cell membrane, and expressed abundantly both GLUT1 and LDH-A. The findings correspond well with the rich existence of lactate
in the genital fluids and strongly suggest the active transport of lactate/pyruvate in the female reproductive tract, which
provides favorable conditions for oocytes, sperms, and zygotes. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|