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| 不同方法检测鸡胴体中沙门菌结果的比较研究 | |
| 引用本文： | 卢行安,段莹,刘颜泓,孙海欧,崔秀云.不同方法检测鸡胴体中沙门菌结果的比较研究[J].中国微生态学杂志,2007,19(3):259-260,262. |
| 作者姓名： | 卢行安 段莹 刘颜泓 孙海欧 崔秀云 |
| 作者单位： | 1. 大连医科大学,辽宁,大连,116027;辽宁出入境检验检疫局,辽宁,大连,116001 2. 大连医科大学,辽宁,大连,116027 3. 大连产品质量监督检验所,辽宁,大连,116023 4. 辽宁出入境检验检疫局,辽宁,大连,116001 |
| 摘 要： | 对鸡胴体淋洗液样品进行沙门菌检测,样品经过前增菌和选择性增菌后,分别采用4种不同的方法进行检测,即普通PCR方法、实时荧光PCR方法、免疫学方法(VIDAS)和传统的微生物检验方法。共检测了56份样品,普通PCR检出阳性样品34份,实时荧光PCR阳性样品36份,VIDAS阳性样品28份;PCR和实时荧光定量PCR均无假阳性和假阴性结果。结果显示该3种检测方法均可以用于鸡胴体中沙门菌的快速检测。 |
| 关 键 词： | 鸡胴体 沙门氏菌 实时荧光PCR |
| 文章编号： | 1005-376X（2007）03-0259-03 |
| 收稿时间： | 2007-05-08 |
| 修稿时间： | 2007-05-08 |
The study on comparison of the microbiological culture, PCR, real-time PCR and VIDAS for detection of Salmonella in whole chicken rinses |
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| LU Xing-an,DUAN Ying,LIU Yan-hong,SUN Hai-ou,CUI Xiu-yun.The study on comparison of the microbiological culture, PCR, real-time PCR and VIDAS for detection of Salmonella in whole chicken rinses[J].Chinese Journal of Microecology,2007,19(3):259-260,262. | |
| Authors: | LU Xing-an DUAN Ying LIU Yan-hong SUN Hai-ou CUI Xiu-yun |
| Institution: | 1. Dalian Medical University ,Dalian 116027, China |
| Abstract: | The aim of our study was to compare the efficiency of different methods for rapid Salmonella detection in whole chicken rinses samples and to optimize the most appropriated method of detection.The microbiological culture,the broth culture-PCR,the real-time PCR and VIDAS methods were used for the detection.Total 56 samples were detected,Salmonella were founded in 34 samples by the PCR,36 positive by real-time PCR,and 28 by VIDAS.The above three detecting method were suitable for the rapid detection of Salmonella in whole chicken rinses. |
| Keywords: | PCR VIDAS |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |