Improved Phycocatalysis of Carotene Production by Flow Cytometry and Cell Sorting |
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| Authors: | Arthur M. Nonomura David M. Coder |
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| Affiliation: | a Research and Development Division, Microbio Resources Inc., San Diego, CAb Marine Biology Research Division, Scripps Institution of Oceanography, University of California, La Jolla, CA, USAc EPICS Division, Coulter Corp., Hialeah, FL, USA |
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| Abstract: | Using flow cytometry, strains of Dunaliella salina that produce large amounts of beta-carotene were sorted from mixed populations. When grown in large outdoor bioreactors, one carotene-producing strain thrived and was phenotypically stable throughout the three year trial. The use of this high-yield strain in industrial-scale processes doubled beta-carotene productivity. Flow cytometry provided a rapid and precise method to identify and isolate algal strains of potential commercial value. |
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| Keywords: | Microalgae Dunaliella strain selection phycocatalysis carotene flow cytometry cell sorting |
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