Cloning and expression of an endo-1,6-beta-D-glucanase gene (neg1) from Neurospora crassa |
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| Authors: | Oyama Seiji Yamagata Youhei Abe Keietsu Nakajima Tasuku |
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| Institution: | Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. |
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| Abstract: | A gene (neg1) encoding an endo-1,6-beta-D-glucanase from Neurospora crassa was cloned. The putative neg1 was 1443-bp long and encoded a mature endo-1,6-beta-D-glucanase protein of 463 amino acids and signal peptide of 17 amino acids. The purified recombinant protein (Neg1) obtained from Escherichia coli showed 1,6-beta-D-glucanase activity. No genes similar in sequence were found in yeasts and fungi. |
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