Immunocytochemical localization of indole-3-acetic acid in primary roots of Zea mays |
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Authors: | L. SHI I. MILLER R. MOORE |
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Affiliation: | Department of Biological Sciences, Wright State University, Dayton, Ohio 45435;Department of Biology, The University of Akron, Akron, Ohio 44325-4703, USA |
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Abstract: | Colloidal gold-labelled antibody was used to localize indole-3-acetic acid in caps of primary roots of Z. mays cv. Kys. Gold particles accumulated on the nucleus, vacuoles, mitochondria, and some dictyosomes and dictyosome-derived vesicles. This is the first localization of indole-3-acetic acid in dictyosomes and dictyosome-derived vesicles and indicates that dictyosomes and vesicles constitute a pathway for indole-3-acetic acid movement in and secretion from root cap cells. Our findings provide cytochemical evidence to support the hypothesis that indole-3-acetic acid plays an important role in root gravitropism. |
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Keywords: | Zea mays, corn indole-3-acetic acid (IAA) colloidal gold immunocytochemistry |
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