Isolation and investigation of anaerobic microorganisms involved in methanol transformation in an underground gas storage facility |
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Authors: | A L Tarasov I A Borzenkov N A Chernykh S S Belyayev |
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Institution: | 1.Winogradsky Institute of Microbiology,Russian Academy of Sciences,Moscow,Russia |
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Abstract: | High methanol and acetate concentrations (up to 12 and 14 g l−1, respectively) were found in water samples collected at different objects of the North Stavropol underground gas storage
facility (UGSF), and significant seasonal variations in the content of these compounds were revealed. The dominant anaerobic
microorganisms isolated from these samples during the study belonged to acetogens, methanogens, and sulfate reducers. The
results of 16S rRNA gene sequencing and analysis of the physiological properties showed that the isolates were close to the
species of Eubacterium limosum, Sporomusa sphaeroides, Methanosarcina barkeri, Methanobacterium formicicum, and Desulfovibrio desulfuricans. The isolated organisms, except for Methanobacterium formicicum, were capable of methylotrophic growth. All strains were characterized by resistance to high methanol concentrations (up
to 40–50 g l−1). Their other energy substrate was hydrogen. The combination of the growth characteristics of these strains (pH, temperature,
and salinity ranges) was shown to correspond to the ecological situation observed in the UGSF. The results of investigation
of the isolated strains suggest that organic acids (acetate, butyrate) found in high concentrations in the initial samples
are metabolic products of the revealed acetogens. Based on the established biological peculiarities of the isolated strains
of methanogens, acetogens, and sulfate-reducing bacteria, these microorganisms may be considered as the main agents of anaerobic
transformation of methanol and some other organic and inorganic compounds in UGSFs. |
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