脑组织特异表达S100B转基因小鼠的建立

目的S100B在多种神经损伤性疾病中高表达,高浓度的S100B蛋白对中枢神经系统具有毒性作用。建立脑组织特异表达S100B转基因小鼠,用于研究该基因在帕金森病（Parkinson＇s disease,PD）发病中的作用。方法ELISA法检测S100B蛋白在α-synuclein A53T转基因小鼠脑组织中的表达情况。构建PDGF-hS100B表达载体,显微注射法建立S100B转基因小鼠。PCR鉴定转基因鼠的基因型,Western blot检测基因表达水平。Rota-rod实验检测S100B转基因鼠的运动协调能力。结果S100B在9月龄和12月龄α-synucleinA53T转基因小鼠脑组织中的表达高于野生型小鼠。建立了2个品系的脑组织特异表达S100B转基因小鼠。与野生型小鼠相比S100B转基因小鼠表现出明显的进行性运动协调能力障碍。结论S100B转基因小鼠的建立为研究该基因在PD中的作用提供了工具。

Establishment of Brain-Specific S100B Transgenic Mice

Objective To establish a brain specific S100B gene transgenic mice and investigate its effect on the development of Parkinson＇s disease（PD）.Methods The expression of S100B protein in the brain of α-synuclein A53T transgenic mice was detected by ELISA.The expression vector of PDGF-hS100B was constructed.The transgenic mice were produced by microinjection and the genotyping was detected by PCR.The expression levels of the transgenic gene were detected by Western blot.Motor coordination of the S100B transgenic and control mice was detected by Rota-rod test.Results The expression of S100B protein in the brain of 9-and 12-month old α-synuclein A53T transgenic mice was higher than that of the wild type mice.Five founders of brain specific PDGF-hS100B transgenic mice were established and two high-level expression lines were identified.Compared with the wild type mice,S100B transgenic mice showed apparently progressive decline in motor coordination assessed by Rota-rod test.Conclusion The transgenic mice with brain specific expression of human S100B gene have been established and it can be used to investigate the function of S100B gene on the development of Parkinson＇s disease.