大鼠心肌缺血再灌注模型构建的改良

目的改良大鼠心肌缺血再灌注模型的构建方法,增强实用性。方法32只SD雄性大鼠随机分为四组（A组：伪手术组;B组：缺血组;C组：缺血30 min再灌注组;D组：缺血60 min再灌注组）。缺血过程在人工机械通气条件下完成,术前术中监测心电图变化,模型成功72 h后抽血进行心肌酶谱分析,并观察左室压变化。结果造模成功率为91%。B、C、D组各心肌酶含量明显高于A组（P〈0.05）,C、D组低于B组（P〈0.05）。B、C、D组左室压值低于A组（P〈0.05）,C、D组高于B组（P〈0.05）。结论改进后的模型制备方法简便易行,成功率高,评价指标符合临床应用。

Improvement of Establishing a Myocardial Ischemic Reperfusion Model in Rats

Objective To improve the method of establishing model in myocardial ischemic reperfusion rat.Methods 32 SD male rats were divided into four groups randomly（Group A：sham group,Group B：myocardial ischemic,Group C：ischemic 30 min and reperfusion,Group D：ischemic 60 min and reperfusion）.Inspected the changing of ECG in preparation /in-operation and rats breathing were finished under the control of artificial respirator in the period of MI.After 72 h of the operation,the levels of myocardial zymogram were determined and the left ventricular pressure was traced.Results Survival rate was 91% after operation.Myocardium zymogram content in the group B,C,D was significantly higher than that of group A（P 0.05）;Myocardium zymogram content in group C、D was lower than that of group B（P 0.05）.The left ventricular pressure in group B,C,D was lower than that of group A（P 0.05）;The left ventricular pressure in group C、D was higher than that of group B（P 0.05）.Conclusion The improving method is convenient,higher survival rate,and the evaluation was according with clinical procedures.