低硒小鼠模型的建立及低硒对心肌的影响

目的建立低硒实验动物模型,观察低硒对心肌的影响。方法利用黑龙江地产酵母配制低硒小鼠饲料,使用配制的小鼠饲料喂养BALB/c幼鼠,经过4个月的喂养,测定血清、肝脏、心肌细胞的硒含量,观察心肌超微结构的变化,测定血清心肌酶的变化。结果利用黑龙江地产酵母配制的低硒饲料,硒含量为0.016 mg/kg,符合低硒标准。BALB/c鼠用该饲料喂养4个月,心肌、肝脏、血清硒含量分别为0.187 mg/kg、0.219 mg/kg、0.241mg/kg,符合低硒诊断标准。观察低硒鼠心肌超微结构,可见心肌细胞线粒体肿胀,细胞核出现了异型性,血清心肌酶较常硒鼠升高。结论利用黑龙江地产酵母成功配制了低硒饲料。经过低硒饲料饲养可建立低硒鼠模型。低硒可以引起BALB/c鼠心肌细胞损伤。

Establishment of Selenium-deficiency Mouse Model and Effects of Selenium-deficiency on Mouse Myocardium

Objective To develop selenium-deficient BALB/c mice by feeding with self-made selenium-deficient diet.Methods／ A selenium-deficient mouse food was prepared with local yeast.Young BALB/c mice were fed with the selenium-deficent diet for over 4 months.Selenium concentrations in the blood,liver,and myocardial tissue of the mice fed with selenium-deficient diet were examined.The myocardial enzymes in the treated mice were examined.The ultrastructures of the treated mice myocardial tissues were also observed.Results The selenium concentration in the mouse food prepared with local yeast was 0.016 mg/kg and the standard of selenium-deficiency.The selenium levels in the serum,liver,and myocardial tissue of BALB/c mice fed with the selenium-deficient diet for 4 months were 0.241 mg/kg,0.219 mg/kg,and 0.187 mg/kg.Swelling mitochondria and heteromorphic nucleus could be observed in the selenium-deficient cardiomyocytes.The level of myocardial enzymes in the sera of the selenium-deficient mice was increased.Conclusions A selenium-deficient mouse diet was successfully developed with local yeast,Selenium-deficient mouse can be prepared by feeding this diet.Selenium-deficiency may cause injuries in cardiomyocyte.