冷拘束应激对大鼠心脏和肝脏HSP70表达及血清中CK、ALT含量的影响

目的通过研究冷拘束应激中大鼠心脏和肝脏组织热休克蛋白（HSP70）表达量以及血清中肌酸激酶（CK）、谷丙转氨酶（ALT）含量的变化,探讨冷拘束应激对心脏和肝脏的影响和差异。方法选取50日龄清洁级大鼠77只,随机分为7组,即0h,0.5h,1.0h,1.5h,2.0h,2.5h,3.0h。采用拘束冷应激法,应激相应时间后,用免疫组织化学方法观察大鼠心脏和肝脏组织HSP70的表达,并检测血清中CK和ALT含量。结果 （1）心脏中HSP70表达量随着应激时间的延长而增加,1.5h组较0h组有明显的增加（P〈0.05）,而2.0h后有极明显的增加（P〈0.01）。（2）肝脏中HSP70表达量随应激时间的延长呈缓慢增加,仅3.0h组有较明显的增加,与0h组、0.5h组及1.0h组比较有显著差异（P〈0.05）。（3）应激开始阶段大鼠血清中的CK含量迅速上升,之后平缓升高;大鼠血清中的ALT含量呈现上升趋势,2.5h以前上升较平缓,2.5h以后急骤升高。结论冷拘束应激可使大鼠心脏中HSP70的表达量和血清中CK含量在较短的时间内显著上升,且随着时间的增加而递增,而肝脏中HSP70的表达量和血清中ALT含量在较短的时间内无明显变化,只有在3.0h时才显著增加,提示心脏对冷拘束应激较敏感,而肝脏对冷拘束应激有较强的耐受性。

The Effect of Cold Restraint Stress on the Expression of Heat Shock Protein in Heart and Liver and the Levels of CK,ALT from Serum in Rats

Objective To explore the effect of cold restraint stress on heart and liver of rats,the expression of HSP70 and the levels of CK and ALT in serum was studied. Methods 77 SD rats of 50 days old were randomly divided into 7 groups,namely 0 h,0. 5 h,1. 0 h,1. 5 h,2. 0 h,2. 5 h,3. 0 h group. The cold-restrained rats lasted the corresponding time,then the distributions of HSP70 in heart and liver tissues were examined by means of immunohistochemistry method and the level of CK and ALT in serum was determined. Results （1）The HSP70 expression in heart increased with stress time 1. 5 h group had an obvious increase compared with the 0 h group （ P 0. 05）,and after 2. 0 h group,the increase was more significant. （2）The HSP70 expression in liver increased slowly with stress time. Only 3. 0 h group had an obvious increase compared with the 0 h,0. 5h and 1. 0h group （P 0. 05）. （3）The level of CK rose quickly at the beginning stage of the stress,then changed smoothly. The level of ALT in serum rose with stress time,during the first 2. 5 h changed smoothly but significantly increased at 2. 5 h group. Conclusion The cold restraint stress could induce the expression of HSP70 in heart and the level of CK in serum markedly increasing in a short time,and increased with stress time,while the expression of HSP70 in liver and the level of ALT in serum had no markedly change in a short time,only after 3 hours the increase became obvious,it suggests that heart is more sensitive to the cold restraint stress,while liver has more powerful resistance.