GnRH—a对大鼠海马和皮质部位parvalbumin表达的影响

目的：研究促性腺激素释放激素类似物（gonadotropin releasing hormone analogue,GnRH-a）对大鼠海马、皮质部位小白蛋白（parvalbumin,PV）表达的影响。方法：采用酶联免疫吸附试验测定血清激素水平,免疫组织化学和图像分析观察大鼠海马、皮质部位PV的表达。结果：①GnRH-a组大鼠血清雌二醇（estradiol,E2）、LH及FSH水平较正常对照组下降,差异有统计学意义（P〈0.05）;与OVX组比较LH、FSH较低,差异有统计学意义（P〈0.05）,但E2差异不显著。②正常情况下,大鼠海马、皮质部位可观察到PV神经元及纤维分布。③GnRH-a组PV细胞数及灰度值低于正常对照组,差异有统计学意义（P〈0.05）,与去卵巢组大鼠组相似;GnRH-a＋E2联合用药后,PV细胞数及灰度值与正常对照组相似。结论：GnRH-a降低大鼠海马、皮质部位神经元中PV的表达,从而影响神经元的功能,这可能与其用药后神经精神副反应有一定关系。

Effect of Gonadotropin Releasing Hormone Analogue on Parvalbumin in Hippocampus and Cerebral Cortex of Rats

Objective：To investigate the effects of gonadotropin releasing hormone analogue（GnRH-a） on change of parvalbumin（PV） immunoreactivities in hippocampus and cerebral cortex of the rats.Methods：ELISA had been used to measure serum hormone levels and immunohistochemistry and image analysis had been used to assay the expression of PV respectively.Results：①Rats serum concentrations of E2 and LH and FSH had decreased in the GnRH-a group compared with the normal group（P〈0.05）;The E2 was simi-lar with that of the OVX group,but concentrations of LH and FSH were significantly lower（P〈0.05）.② PV expressed in hippocampus and cerebral cortex of normal rats.Compared with the saline treated normal group,the PV expression in hippocampus and cerebral cortex was significantly decreased after GnRH-a treated（P〈0.05）,which were similar with the results in the OVX rats.③ Adding E2 could reverse the changes caused by GnRH-a,which was similar to normal rats.Conclusion GnRH-a declined PV expression in hippocampus and cerebral cortex of the rats,thus change the function of neurons,which might be associated with the GnRH-a neuropsychosis side effects.