基于SSR标记构建葡萄种质资源分子身份证 |
| |
引用本文: | 杜晶晶;刘国银;魏军亚;刘德兵;杨小振. 基于SSR标记构建葡萄种质资源分子身份证[J]. 植物研究, 2013, 33(2): 232-237 |
| |
作者姓名: | 杜晶晶;刘国银;魏军亚;刘德兵;杨小振 |
| |
作者单位: | 1.海南大学园艺园林学院,海口 570228;;2.中国热带农业科学院热带作物品种资源研究所,农业部热带作物种质资源利用重点开放实验室,儋州571737;;3.西北农林科技大学园艺学院,杨凌 712100 |
| |
摘 要: | 以国家葡萄品种资源圃内保存的80份葡萄种质为试材,对构建葡萄种质分子身份证的方法进行探讨研究。利用筛选后的SSR标记对供试品种进行区分,然后根据引物对不同品种扩增条带分子量的大小进行编码。从62对引物中筛选出来自葡萄19条染色体上的28对SSR引物,在供试种质间共检测出等位基因169个,每对引物平均检测到等位基因数为6.0个。将等位基因赋值后仅用9对引物构建了供试种质的分子身份证编码,平均每对引物区分种质达8.9份,达到了区分品种更加简洁明了,用最少的引物区分不同品种的目的,表明SSR标记技术可有效用于建立葡萄种质资源分子身份证。
|
关 键 词: | 葡萄;SSR;分子身份证 |
Establishment of Molecular ID for Grape Germplasm Based on SSR Markers |
| |
Affiliation: | 1.College of Horticulture and Forestry,Hainan University,Haikou 570228;2.Tropical Crops Genetic Resources Institute of Chinese Academy of Tropical Agriculture Sciences,Key Tropical Crop Germplasm Utilization, Ministry of Agriculture,Danzhou 571737;3.College of Horticulture,Northwest A&;F University,Yangling 712100 |
| |
Abstract: | Using a total of 80 grape germplasms selected from national grape repository of Zhengzhou as the material, the methods of establishing the grape germplasms molecular ID were studied. Germplasms were distinguished by the selected SSR markers, and then coded the size of augmentation strip molecular based on the different germplasms. The results showed that after screening of the 62 primers, 169 alleles were detected using 28 selected SSR markers located on each chromosome of grape, with a mean value of 6.0 alleles/locus. After alleles assignment, there were only 9 pairs of primers selected out for the construction of germplasms molecular ID with an average value of 8.9 germplasms/primers. The purpose of distinguishing the variety of grape was achieved by a minimum of primers. The results suggested SSR marker techniques can be used for establishing molecular identity of grape germplasm. |
| |
Keywords: | grape(Vitis) SSR molecular ID |
|
| 点击此处可从《植物研究》浏览原始摘要信息 |