Formation of embryoid bodies from mouse embryonic stem cells cultured on silicon-coated surfaces |
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| Authors: | Fardin Fathi Taki Altiraihi Seyed Javad Mowla Mansoreh Movahedin |
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| Institution: | (1) Department of Anatomy, KDRC, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran;(2) Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran;(3) Department of Genetics, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran |
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| Abstract: | Embryoid bodies (EBs) are primitive embryonic structures derived from differentiating embryonic stem cells (ESCs). Many techniques
have been used to obtain EBs. Improving the technique of EB formation can help in achieving better results in ESCs differentiation
into neurons, myocardiocytes, haemopoeitic cells, and others. We evaluated the use of Sigmacote™ as a hydrophobic substrate
to improve EB formation. CCE and P19 cell lines were used to obtain EBs and retinoic acid was used to induce neural differentiation.
The results revealed that Sigmacote™, as a hydrophobic substrate, can improve EB formation from ESCs. Our results demonstrate
that the silicon-coating of glass petri dishes by Sigmacote™ is an easy and reproducible technique to enhance EB formation
from murine ESCs and EC cells. |
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| Keywords: | Embryoid body Hydrophobic substrate Sigmacote P19 cell line CCE cell line |
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