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Rapid detection of methicillin resistance in teicoplanin-resistant coagulase-negative staphylococci by a penicillin-binding protein 2' latex agglutination method |
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| Authors: | Knausz Márta Ghidán Agoston Grossato Anna Rozgonyi Ferenc |
| Institution: | aClinical Microbiology, Aladár Petz County Teaching Hospital, 13 Zrinyi Street, 9024, Gy?r, Hungary bInstitute of Medical Microbiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary cInstitute of Histology, Microbiology and Biotechnology, University of Padova, Padova, Italy |
| Abstract: | Thirty-five clinical isolates of coagulase-negative staphylococci with decreased glycopeptide sensitivity were examined by a penicillin-binding protein (PBP2′) latex agglutination (LA) test and were compared to the detection of the mecA gene by PCR, and oxacillin susceptibility determined minimum inhibitory concentrations. The latex test demonstrated high sensitivity and specificity for detecting methicillin resistance in coagulase-negative staphylococci after PBP2′ induction with oxacillin. |
| Keywords: | Penicillin binding protein Teicoplanin resistance |
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