Characterization of species-specific repeated DNA sequences from B. nigra |
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| Authors: | Vibha Gupta G LakshmiSita M S Shaila V Jagannathan Malathi S Lakshmikumaran |
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| Institution: | (1) Tata Energy Research Institute, 90 Jorbagh, 110003 New Delhi, India;(2) Microbiology and Cell Biology Department, Indian Institute of Science, 560012 Bangalore, India |
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| Abstract: | Summary The construction and characterization of two genome-specific recombinant DNA clones from B. nigra are described. Southern analysis showed that the two clones belong to a dispersed repeat family. They differ from each other in their length, distribution and sequence, though the average GC content is nearly the same (45%). These B genome-specific repeats have been used to analyse the phylogenetic relationships between cultivated and wild species of the family Brassicaceae. |
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| Keywords: | Repeated DNA Brassica species Genome specificity Phylogenetic studies |
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