Repression of c-myc is necessary but not sufficient for terminal differentiation of B lymphocytes in vitro |
| |
Authors: | Lin K I Lin Y Calame K |
| |
Affiliation: | Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA. |
| |
Abstract: | The importance of c-myc as a target of the Blimp-1 repressor has been studied in BCL-1 cells, in which Blimp-1 is sufficient to trigger terminal B-cell differentiation. Our data show that Blimp-1-dependent repression of c-myc is required for BCL-1 differentiation, since constitutive expression of c-Myc blocked differentiation. Furthermore, ectopic expression of cyclin E mimicked the effects of c-Myc on both proliferation and differentiation, indicating that the ability of c-Myc to drive proliferation is responsible for blocking BCL-1 differentiation. However, inhibition of c-Myc by a dominant negative form was not sufficient to drive BCL-1 differentiation. Thus, during Blimp-1-dependent plasma cell differentiation, repression of c-myc is necessary but not sufficient, demonstrating the existence of additional Blimp-1 target genes. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|