Nucleotide sequence, heterologous expression and novel purification of DNA ligase from Bacillus stearothermophilus(1). |
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| Authors: | J A Brannigan S R Ashford A J Doherty D J Timson D B Wigley |
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| Institution: | Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK. |
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| Abstract: | The gene for DNA ligase (EC 6.5.1.2) from thermophilic bacterium Bacillus stearothermophilus NCA1503 has been cloned and the complete nucleotide sequence determined. The ligase gene encodes a protein 670 amino acids in length. The gene was overexpressed in Escherichia coli and the enzyme has been purified to homogeneity. Preliminary characterisation confirms that it is a thermostable, NAD(+)-dependent DNA ligase. |
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