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| 拟南芥鼠李糖合成酶基因AtRHM1启动子区葡萄糖应答调控元件的鉴定 | |
| 引用本文： | 季秦梅,王金凤,姜凌,沈颂东,范云六,张春义.拟南芥鼠李糖合成酶基因AtRHM1启动子区葡萄糖应答调控元件的鉴定[J].生物工程学报,2008,24(9):1531-1537. |
| 作者姓名： | 季秦梅 王金凤 姜凌 沈颂东 范云六 张春义 |
| 作者单位： | 1. 苏州大学生命科学学院,苏州,215006;中国农业科学院生物技术研究所植物生物技术研究室,北京,100081 2. 中国农业科学院生物技术研究所植物生物技术研究室,北京,100081;国家农作物基因资源与遗传改良重大科学工程,北京,100081 3. 苏州大学生命科学学院,苏州,215006 |
| 摘 要： | 植物中,UDP-L-鼠李糖是细胞壁骨架的主要成分,由鼠李糖合成酶催化底物UDP-α<,-D->葡萄糖合成.本实验从拟南芥基因组中分离了鼠李糖合成酶基因AtRHM1 1058bp的启动子序列并对启动子5'端进行了不同长度的缺失.将全长启动子及不同缺失启动子与GUS报告基因进行融合后转化野生型拟南芥,获得了一系列转基因植株.启动子缺失分析结果表明,AtRHM1基因在转录水平上受葡萄糖的诱导,参与葡萄糖应答反应的顺式调控元件位于启动子的-931 bp～-752bp区域. |
| 关 键 词： | 鼠李糖合成酶基因(RHM1) 启动子缺失 葡萄糖应答 顺式调控元件 |
| 收稿时间： | 2008/1/11 0:00:00 |
Identification of Glucose-responsive Elements in the Promoter of UDP-L-rhamnose Biosynthesis Gene RHM1 in Arabidopsis thaliana |
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| Qinmei Ji,Jinfeng Wang,Ling Jiang,Songdong Shen,Yunliu Fan and Chunyi Zhang.Identification of Glucose-responsive Elements in the Promoter of UDP-L-rhamnose Biosynthesis Gene RHM1 in Arabidopsis thaliana[J].Chinese Journal of Biotechnology,2008,24(9):1531-1537. | |
| Authors: | Qinmei Ji Jinfeng Wang Ling Jiang Songdong Shen Yunliu Fan and Chunyi Zhang |
| Institution: | School of Life Sciences, Soochow University, Suzhou 215006, China; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China; National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, Beijing 100081, China;Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China; National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, Beijing 100081, China;School of Life Sciences, Soochow University, Suzhou 215006, China;Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China; National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, Beijing 100081, China;Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China; National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, Beijing 100081, China |
| Abstract: | |
| Keywords: | rhamnose sythnase gene (RHM1) promoter deletion glucose response cis-regulatory elements |
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