Localization of actin in the electrocyte of Electrophorus electricus L. |
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Authors: | Marília Taffarel Marcos Farina de Souza Dr Raul Dodsworth Machado Wanderley de Souza |
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Institution: | (1) Departamento de Histologia e Embriologia, Laboratório de Microscopia Electrônica e Laboratório de Ultraestrutura Celular, Instituto de Biofisica da Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil;(2) Laboratório de Microscopia Electrônica, Instituto de Biofisica UF RJ, Ilha do Fundâo, 21941 Rio de Janeiro, Brasil |
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Abstract: | Summary The cytoplasm of the electrocyte of Electrophorus electricus possesses a meshwork of 7-nm thick filaments distributed throughout the cell. Observation of stereopairs of transmission electron micrographs shows association of the filaments with the plasma membrane and the membranes of cytoplasmic organelles. Intense fluorescence, indicative of the presence of actin, was observed in the cytoplasm of electrocytes incubated in the presence of NBD-phallacidin or anti-actin antibodies. |
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Keywords: | Electrocyte Electric eel Actin filaments Cytoskeleton Electrophorus electricus |
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