Identification of 4-chloroindole-3-acetic acid and indole-3-aldehyde in seeds of Pinus sylvestris |
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Authors: | Arild Ernstsen Göran Sandberg |
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Institution: | Dept of Forest Genetics and Plant Physiology, The Swedish Univ. of Agricultural Sciences, S-901 83 Umeå, Sweden. |
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Abstract: | 4-Chloroindole-3-acetic acid (4-Cl-IAA) and indole-3-aldehyde (IAId) have been characterized as endogenous constituents in seeds of Pinus sylvestris L. by gas chromatography-mass spectrometry. Quantitative estimates indicate that immature seeds contained 640 pg 4-Cl-IAA (g fresh weight)-1 while mature seeds contained 340 pg (g dry weight)-1. 4-Cl-IAA could not be detected in seeds five days after germination. The content of IAld increased from 127 pg (g dry weight)-1 in mature seeds to 315 pg (g dry weight)-1 after five days of germination. |
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Keywords: | Auxins gas chromatography-mass spectrometry Scots pine seed development seed germination |
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