Effects of temperature and growth phase on lipid and biochemical composition of Isochrysis galbana TK1 |
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Authors: | C J Zhu Y K Lee T M Chao |
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Institution: | (1) Department of Microbiology, Faculty of Medicine, National University of Singapore, Lower Kent Ridge Road, Singapore, 119260;(2) Mariculture and Foodfish Section, Primary Production Department, Nicoll Drive, Changi Point, Singapore, 498989 |
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Abstract: | The lipid and biochemical composition of the haptophyte Isochrysis galbana TK1 was examined. Cultures were grown at 15 °C
and 30 °C, and harvested in the exponential and early stationary growth phases. Carbohydrate and protein content varied at
the two culture temperatures and growth phases. The highest protein content was found at the exponential growth phase at 15
°C, and the highest carbohydrate content was found at the stationary phase at the same culture temperature. Lipid accumulated
in the stationary growth phase and its content was higher at 30 °C than at 15 °C regardless of the growth phase. The neutral
lipids were the major class of lipid found in all the cultures. The stationary phase culture had a higher proportion of neutral
lipids than the exponential phase culture and the proportion decreased slightly when culture temperature was increased from
15 °C to 30 °C. Phospholipid levels remained constant at the two temperatures, but slightly decreased in the stationary phase.
Glycolipids in the exponentially growing cells were higher than those from stationary growth phase and increased with temperature.
Polyunsaturated fatty acids (PUFAs) predominated in glycolipids and phospholipids. Cells grown at 15 °C contained higher proportion
of 18:3 (n–3) and 22:6 (n–3) with a corresponding decrease in 18:2 (n–6), monounsaturated and saturated fatty acids.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | Isochrysis galbana lipid biochemical composition fatty acid temperature growthphase |
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