Structural studies on the cytochrome c oxidase proton pump using a spin-label probe

The stimulation of sodium transport by aldosterone in target tissues requires the synthesis of both mRNA and proteins. Aldosterone-induced mRNA and proteins have been demonstrated in toad urinary bladder and rat kidney. We have isolated total RNA and poly(A)-containing RNA from hormone-treated and untreated toad bladder mucosal cells for translation in a rabbit reticulocyte lysate system. Aldosterone-induced proteins synthesized in this system have physical properties similar to those of aldosterone-induced proteins synthesized in the intact toad bladder.