巴马小型猪肝硬化前后肠道乳杆菌的比较研究

目的比较巴马小型猪诱导型肝硬化造模前(正常猪)和造模成功后(肝硬化猪)肠道乳杆菌的变化情况,探讨小型猪肝硬化模型在肝硬化肠道微生态研究中的适用性。方法收集肝硬化造模前和CCl4诱导肝硬化造模成功后巴马小型猪的新鲜粪便,提取粪便总菌DNA,用乳杆菌特异性引物进行PCR扩增,对扩增产物进行变性梯度凝胶电泳(Denaturing Gradient Gel-Electrophoresis,DGGE),即用PCR-DGGE分析巴马小型猪肝硬化前后肠道乳杆菌的相似性和多样性。结果聚类分析和主成分分析显示巴马小型猪肝硬化前(正常猪)和肝硬化后混杂排列,无明显界限;多样性数据分析显示巴马小型猪肝硬化前后肠道乳杆菌的丰富度(S)、微生物区系Shannon-Wiener指数(H′)和均匀度(E)差异均无统计学意义(P0.05)。结论巴马小型猪肝硬化后肠道乳杆菌与正常猪比较差异无统计学意义。

Comparative analysis of Lactobacillus-specific molecular profile of intestinal microflora between normal and cirrhosis Bama miniature pigs by PCR-DGGE

Objective To analyze and compare the richness,Shannon-Wiener index and evenness of Lactobacillus community in intestinal microflora of normal and cirrhosis Bama miniature pigs.Method Lactobacillus-specific Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis（PCR-DGGE） analysis of intestinal microflora was performed.Total bacterial DNAs of the fecal samples were extracted and the total DNA samples subjected to Lactobacillus-specific 16S rDNA-based PCR-DGGE.The molecular DGGE profile was subjected to UPGMA and Principal Component analysis using Quantity One（Bio-rad） and origionab 7.0 software respectively.Result No remarkable differences were observed in the richness,Shannon-Wiener index and evenness of Lactobacillus in intestinal microflora between normal and cirrhosis Bama miniature pigs by statistical analysis（P0.05）.Principal component analysis also indicated that cirrhosis did not alter the Lactobacillus community in intestinal microflora of Bama miniature pigs.Conclusion No remarkable differences was observed in the Lactobacillus-specific DGGE profile between cirrhosis and normal Bama miniature pigs.