T cell activation by purified, soluble, class I MHC molecules. Requirement for polyvalency |
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Authors: | J McCluskey L F Boyd P F Highet J Inman D H Margulies |
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Institution: | Molecular Biology Section, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892. |
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Abstract: | To examine the nature of the interaction of the TCR with the MHC class I Ag, we have studied the stimulation requirements of an H-2Dd-reactive T cell hybridoma, using a homogeneous, purified preparation of a molecularly engineered soluble counterpart of the class I Ag, H-2Dd/Q10b. We demonstrate that this monovalent, soluble MHC Ag is incapable of stimulating the release of IL-2 from this T cell hybridoma. However, the same preparation of the purified protein can elicit a dose-dependent response when made multivalent either by covalent coupling to soluble, high m.w. dextran or to agarose beads, or by adsorption to polystyrene tissue culture plates. |
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