Converting Sendai virus into a specific fusogen whose cell target can be selected |
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| Authors: | O Martinez J Kimura C Henry L Wofsy |
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| Affiliation: | 1. Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean China, 5 Yushan Road, Qingdao 266003, China;2. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, No.1 Wenhai Road, Aoshanwei Town, Jimo, Qingdao 266071, China;1. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Science, National Chromatographic Research and Analysis Center, 457 Zhongshan Road, Dalian 116023, China;2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China;1. Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino, Italy;2. Department of Veterinary Medical Sciences, Alma Mater Studiorum, Università di Bologna, Ozzano dell’Emilia, BO, Italy;3. Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, PD, Italy;4. Laboratoire Frank Duncombe LABEO, Caen, France;1. Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea;2. Department of Chemistry, University of Colombo, Colombo 03, Sri Lanka;1. Department of Aqualife Medicine, Chonnam National University, Yeosu, Republic of Korea;2. Institute of Marine Biotechnology, Pukyong National University, Busan, Republic of Korea |
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| Abstract: | Covalent intermolecular hybrids of Fab anti-hemagglutinin-neuraminidase (HN) monoclonal antibody and avidin were prepared and characterized. These conjugates were used to block and redirect the fusion activity of Sendai virus (SV). After incubation of SV with Fab anti-HN: avidin conjugate on ice for 1-2 h, the SV fused only those P815 or BW5147 cells which were labeled with biotin-modified anti-cell surface immunoglobulin. The levels of cell-cell fusion obtained were at least as high as those achieved with unmodified SV and unlabeled P815 or BW5147 cells. These results demonstrate that it is possible to block the normal agglutinating activity of the HN molecules of SV and to introduce a new cell recognition feature without negating the fusogenic potential of the virus. Such an approach may be useful in harnessing the fusion activity of SV to a targeted delivery system for microinjection of macromolecules into selected cell populations. |
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