Endogenous factors affecting arachidonic acid metabolism: I. Biosynthesis of prostacyclin and prostaglandins by carrageenin granulomas of rats |
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Authors: | JA Spawiski B Wojtaszek J
wies RJ Gryglewski |
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Institution: | Department of Pharmacology, Copernicus Medical Academy, 16 Grzegórzecka Street, 31-531 Kraków, Poland |
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Abstract: | Arachidonic acid was converted by incubated slices of the rat carrageenin granuloma to prostacyclin (PGI2), prostaglandins (PGs) E2 and F2∞ as detected by bioassay and radiochemical assay. PGI2 was the major product of arachidonic acid metabolism in the granuloma slices. PGI2 and PGE2 formation was dependent on the concentration of the substrate and on the age of the granuloma. Slices obtained from 5-day old granulomas produced significantly more PGI2 than slices prepared from 3-day old or 8- to 9-day old granulomas while PGE2 generation was not dependent on the stage of the development of the granuloma. Homogenates of granuloma tissue hardly converted arachidonic acid to PGI2 at all. This was probably due to the presence of an non- dialysable and heat labile material which, when partially isolated, inhibited PGI2 production by bovine aortic microsomes. |
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