Participation of alkaline phosphatase in the active transport of phosphates in brush border membrane vesicles |
| |
| Authors: | M Letellier G E Plante N Brière C PetitClerc |
| |
| Affiliation: | Laboratoire de Pharmacologie et de Toxicologie Fondamentales du Centre National de la Recherche Scientifique 205 route de Narbonne, 31400 Toulouse, France |
| |
| Abstract: | This paper reports the separation and preliminary characterization of the products formed by the reaction of the antitumor compound cis-Pt(NH3)2Cl2 with DNA. Electrophoresis of the acid hydrolysed platinum-DNA complex gave a profile of platinum concentration which contained 5 peaks whose relative intensities varied with the amount of cis-Pt(NH3)2Cl2 fixed on the DNA. Similar analysis of the products formed between DNA and trans-Pt(NH3)2Cl2 or [Pt(dien)Cl]Cl, which are not active antitumor agents, indicated that these compounds bound to DNA in a different manner than cis-Pt(NH3)2Cl2. DNA isolated from Escherichia coli which had been treated with cis-Pt(NH3)2Cl2 or [Pt(dien)Cl]Cl did not give the same electrophoresis profiles as the corresponding platinum-DNA complexes formed in vitro. |
| |
| Keywords: | PDD dien molar ratio of platinum covalently bound to DNA (platinum/DNA-nucleotide) CD circular dichroism UV ultraviolet |
| 本文献已被 ScienceDirect 等数据库收录！ |
