Reduced evolvability of <Emphasis Type="Italic">Escherichia coli</Emphasis> MDS42, an IS-less cellular chassis for molecular and synthetic biology applications |
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| Authors: | Kinga Umenhoffer Tamás Fehér Gabriella Balikó Ferhan Ayaydin János Pósfai Frederick R Blattner György Pósfai |
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| Institution: | (1) Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, 62 Temesvari krt, H6726 Szeged, Hungary;(2) Cellular Imaging Laboratory, Biological Research Center of the Hungarian Academy of Sciences, 62 Temesvari krt, H6726 Szeged, Hungary;(3) New England Biolabs, Inc, 240 County Rd, 01938 Ipswitch, MA, USA;(4) Scarab Genomics LLC, 1202 Ann Str, 53713 Madison, WI, USA;(5) Department of Genetics, University of Wisconsin, 425-G Henry Mall, 53706 Madison, WI, USA |
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| Abstract: | Background Evolvability is an intrinsic feature of all living cells. However, newly emerging, evolved features can be undesirable when
genetic circuits, designed and fabricated by rational, synthetic biological approaches, are installed in the cell. Streamlined-genome
E. coli MDS42 is free of mutation-generating IS elements, and can serve as a host with reduced evolutionary potential. |
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