Expression of endostatin mediated by a novel non-viral delivery system inhibits human umbilical vein endothelial cells in vitro |
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| Authors: | Chunmei Zhang Xueju Zhang Chunbo Liu Junfeng Wang Xinghan Liu Hulun Li Jinghua Wang Changjun Wu |
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| Institution: | (1) Department of Ultrasound, First Affiliated Hospital of Harbin Medical University, 150001 Harbin, China;(2) Department of Biochemistry and Molecular Biology, Harbin Medical University, The Key Laboratory of Heilongjiang Provincial Biomedical Engineering, 150086 Harbin, China;(3) Department of Neurobiology, Harbin Medical University, 150086 Harbin, China; |
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| Abstract: | Ultrasound (US)-mediated microbubble destruction is recognized to have considerable potential for gene delivery, whereas,
there is few report of its effect on enhancing liposomal transfection. In this study, we used pIRES2-EGFP/hES containing human
endostatin (hES) cDNA as target gene to test the hypothesis that US exposure with microbubbles could improve liposomal transfection,
and to investigate the possibility of intracellular delivery of ES gene using this method. Under the controlled US exposure
condition with microbubbles, the plasmid:liposome was transferred into COS-7 cells. The transfection rate, the expression
of endostatin and the inhibition effect of transfection-endostatin on endothelial cells were assessed. The results revealed
that US-mediated microbubble destruction together with liposome could significantly enhance gene transfection without obvious
cell damage. By this means, endostatin gene could be efficiently transferred into COS-7 cells and expressed. The transfection-endostatin
could inhibit endothelial proliferation and migration, which suggests that the non-viral method might be useful in anti-angiogenesis
therapy in the future. |
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