Monitoring fermentation parameters during phytase production in column-type bioreactor using a new data acquisition system |
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Authors: | Michele Rigon Spier Adenise Lorenci Woiciechowski Luiz Alberto Junior Letti Gessiel Newton Scheidt Wilerson Sturm Jose Angel Rodriguez-León Julio César de Carvalho Dario Eduardo Amaral Dergint Carlos Ricardo Soccol |
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Institution: | 1. Bioprocess Engineering and Biotechnology Department, Federal University of Parana, Curitiba, Paraná, Brazil 3. UFPR, Av. Cel. Fco H. dos Santos, 100, 81531-990, Curitiba, PR, Brazil 2. Federal Technological University of Parana State, UTFPR, Curitiba, Paraná, Brazil
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Abstract: | Fermentation parameters for phytase production in column-type bioreactor were monitored using a new data acquisition system. There are a number of studies reporting phytase production in flasks, but a lack of data about microorganism respiration behaviour during phytase production using column bioreactor. The objectives of this work were the monitoration of fermentation parameters during phytase production and its relation with fungal growth and forced air. Phytase production by A. niger FS3 increased with forced air. The O2 consumption and CO2 production during solid-state fermentation were monitored by sensors (in the bottom and top of the columns) linked to controllers, recorded by acquisition software and processed by Fersol2® software tool. Phytase synthesis was associated with fungal growth. Therefore, phytase could be used to estimate FS3 biomass formed in citric pulp degradation. |
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