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Monitoring fermentation parameters during phytase production in column-type bioreactor using a new data acquisition system
Authors:Michele Rigon Spier  Adenise Lorenci Woiciechowski  Luiz Alberto Junior Letti  Gessiel Newton Scheidt  Wilerson Sturm  Jose Angel Rodriguez-León  Julio César de Carvalho  Dario Eduardo Amaral Dergint  Carlos Ricardo Soccol
Institution:1. Bioprocess Engineering and Biotechnology Department, Federal University of Parana, Curitiba, Paraná, Brazil
3. UFPR, Av. Cel. Fco H. dos Santos, 100, 81531-990, Curitiba, PR, Brazil
2. Federal Technological University of Parana State, UTFPR, Curitiba, Paraná, Brazil
Abstract:Fermentation parameters for phytase production in column-type bioreactor were monitored using a new data acquisition system. There are a number of studies reporting phytase production in flasks, but a lack of data about microorganism respiration behaviour during phytase production using column bioreactor. The objectives of this work were the monitoration of fermentation parameters during phytase production and its relation with fungal growth and forced air. Phytase production by A. niger FS3 increased with forced air. The O2 consumption and CO2 production during solid-state fermentation were monitored by sensors (in the bottom and top of the columns) linked to controllers, recorded by acquisition software and processed by Fersol2® software tool. Phytase synthesis was associated with fungal growth. Therefore, phytase could be used to estimate FS3 biomass formed in citric pulp degradation.
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